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MEM-NEAA, Earle's Salts Base, with Non-Essential Amino Acids, without L-Glutamine (500 ml)

MEM-NEAA,  Earle's Salts Base, with Non-Essential Amino Acids, without L-Glutamine (500 ml)
MEM-NEAA, Earle's Salts Base, with Non-Essential Amino Acids, without L-Glutamine (500 ml)

MEM-Eagle with Non-Essential Amino Acids (MEM-NEAA), without L-Glutamine is one of the most widely utilized of all synthetic growth media. MEM has been used for the cultivation of a wide variety of cells grown in monolayers. Optional supplementation of Non-Essential Amino Acids (NEAA's) to the formulations that incorporate either Hank's or Eagle's salts has broadened the utility of this medium. 

This MEM is modified as follows:

With

  • Earle’s Salts
  • Non-Essential Amino Acids
  • Sodium Bicarbonate
  • Phenol Red

Without:

  • L-Glutamine
Components Concentration (mg/L)
INORGANIC SALTS:
Calcium Chloride (CaCl2) (Anhyd.) 200.00
Potassium Chloride (KCl) 400.00
Magnesium Sulfate (MgSO4) 97.67
Sodium Chloride (NaCl) 6800.00
Sodium Bicarbonate (NaHCO3) 2200.00
Sodium Phosphate-H2O (NaH2PO4-H2O) 140.00
OTHER COMPONENTS:
D-Glucose 1000.00
Phenol Red 10.00
AMINO ACIDS:
L-Alanine  8.9
L-Arginine-HCl 126.00
L-Asparagine-H2O  15.0
L-Aspartic acid  13.3
L-Cystine 24.00
L-Glutamic acid  14.7
Glycine 7.5 
L-Histidine-HCl-H2O 42.00
L-Isoleucine 52.00
L-Leucine 52.00
L-Lysine-HCl 72.50
L-Methionine 15.00
L-Phenylalanine 32.00
L-Proline  11.5
L-Serine  10.5
L-Threonine 48.00
L-Tryptophan 10.00
L-Tyrosine 36.00
L-Valine 46.00
VITAMINS:
D-Ca Pantothenate 1.00
MEM
NEAA Yes
Type Earle
Format Liquid
Glutamine No glutamine
Phenol Red Yes

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  • Model 01-040-1A
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