DMEM: Nutrient Mixture F-12 (Ham's) (1:1) without L-Glutamine, with Sodium Bicarbonate, with Hepes 15mM, with Sodium (500 ml)

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and fibroblasts. 
This DMEM/F-12 ismodified as follows:

With

• Sodium Bicarbonate
• Sodium Pyruvate
• HEPES

Without:

• L-Glutamine