Lumitein™ Protein Gel Stain 100X (2 ml)

Lumitein™ is a luminescent dye designed for detecting proteins in SDS polyacrylamide (SDS-PAGE) gels. It can also be used to detect proteins in native PAGE gels after an additional SDS incubation step. The stain combines excellent sensitivity, exceptional user-friendliness and compatibility with common instruments and downstream analysis.

Lumitein™ is a luminescent dye designed for detecting proteins in SDS polyacrylamide (SDS-PAGE) gels. It can also be used to detect proteins in native PAGE gels after an additional SDS incubation step. The stain combines excellent sensitivity, exceptional user-friendliness and compatibility with common instruments and downstream analysis. It is as sensitive as the best silver stain by detecting 1 ng or less protein (Figure 1). Unlike silver stain, however, Lumitein™ has a linear detection range of at least 3 orders of magnitude. It is among the simplest protein gel stain by staining protein in gels in 90 minutes or less time without a separate fixation step. Lumitein™ has an excitation spectrum that makes detection possible with either a simple UV box or a high-end laser scanner. Moreover, protein gel staining with Lumitein™ is compatible with downstream protein analyses such as mass spectrometry and Edman-based sequencing (Figure 2).

Lumitein™ Protein Gel Stain 100X is an economical concentrated stock solution. For a fast, easy, and non-toxic alternative to Lumitein™ for protein gel staining, see our One-Step Lumitein™ and One-Step Blue™ Protein Gel Stains.

Features

1. Highly Sensitive: At least as sensitive as silver stain by detecting < 1 ng protein.
   
2. Simple & Fast: Fixation and staining in a single 30-90 minute step, followed by a simple rinse in water.
   
3. Very Low Background
   
4. Instrument Compatibility: Image with 300 nm UV gel box (EtBr filter), Dark Reader, or a laser scanner.
   
5. Wide Linear Detection Range: At least three orders of magnitude.
   
6. Compatible with Downstream Analysis: Compatible with MS and sequencing.
   
7. Highly Stable: Both 100X and 1X solutions are stable at room temperature.
   
8. Economical: 100X concentrate with reduced manufacturing and shipping costs

Download User Manual