For use in short-term cultivation of primary bone marrow cells for chromosome evaluationBIO-MARROW™ Medium is intended for use in short-term cultivation of primary bone marrow cells for chromosome evaluation. The medium is based on RPMI 1640 basal medium supplemented with L-Glutamine, fetal calf ser..
For use in short-term cultivation of primary bone marrow cells for chromosome evaluationBIO-MARROW™ Medium is intended for use in short-term cultivation of primary bone marrow cells for chromosome evaluation. The medium is based on RPMI 1640 basal medium supplemented with L-Glutamine, fetal calf ser..
Peripheral Blood Karyotyping medium is specifically optimized for short-term culture of peripheral blood lymphocytes for chromosome analysis. This formulation consists of a RPMI medium supplemented with serum, L-glutamine, antibiotics (gentamicin) and Phytohemagglutinin M (PHA-M). The PHA-M potency ..
Peripheral Blood Karyotyping medium is specifically optimized for short-term culture of peripheral blood lymphocytes for chromosome analysis. This formulation consists of a RPMI medium supplemented with serum, L-glutamine, antibiotics (gentamicin) and Phytohemagglutinin M (PHA-M). The PHA-M potency ..
Optimized medium for short-term cultivation of peripheral blood lymphocytes for chromosome analysisBiological Industries' BIO-PB™ Karyotyping Medium is specifically optimized for short-term cultivation of peripheral blood lymphocytes for chromosome analysis. BIO-PB™ Karyotyping Medium is based on RP..
Optimized medium for short-term cultivation of peripheral blood lymphocytes for chromosome analysisBiological Industries' BIO-PB™ Karyotyping Medium is specifically optimized for short-term cultivation of peripheral blood lymphocytes for chromosome analysis. BIO-PB™ Karyotyping Medium is based on RP..
The blood cell karyotyping method was developed to provide information about chromosomal abnormalities. Lymphocyte cells do not normally undergo subsequent cell divisions. In the presence of a mitogen, lymphocytes are stimulated to enter into mitosis by DNA replication. After 48-72 hours, a mitotic ..
Colcemid, N-deacetyl-N-Methylcolchicine, is related to colchicine, but animal studies found it to be much less toxic. Colcemid arrests mitotic cultured cells in metaphase and it should be treated carefully, since it is mutagenic, tumorigenic, and teratogenic. Colcemid Solution from Biological Indust..
Colchicine is a major plant alkaloid from Colchicum autumnale L. of the family Liliaceae. It is also commonly known as the Meadow Saffron.This plant extract is also found in other Colchicum spp. and is highly poisonous. It is used as a Cell Synchronization Agent (CSA) that, at appropriate dilutions,..
Phytohaemagglutinin-M (PHA-M) is used for the stimulation of cell proliferation in lymphocyte culture. Phytohaemagglutinin (or Phytohemagglutinin) is a lectin extracted from red kidney beans (Phaseolus vulgaris). The protein consists of two molecular species, a leucoagglutinin (PHA-L) and an erythro..
Hypotonic solution of potassium chloride in water for use in the preparation of blood lymphocyte chromosomes - the hypotonic treatment causes the cells to swell...
A major step in harvesting cells for chromosome karyotyping is treatment with a hypotonic saline solution to increase cell volume. Sodium Citrate Solution is a hypotonic solution, which work by creating a concentration gradient across the cytoplasmic membrane and water then rushes in by active trans..