Nitrotetrazolium Blue (NBT) is used in combination with 5-bromo-4-chloro-3-indoxyl phosphate (BCIP) to detect alkaline phosphatase activity in blotting techniques and in situ hybridization chemistry. BCIP acts as the substrate for alkaline phosphatase, and the oxidizing reagent NBT enhances the purp..
X-phos; 5-Bromo-4-chloro-3-indoxyl phosphate, p-toluidine saltMOLECULAR BIOLOGY GRADEC8H6BrClNO4P·C7H9NMW: 433.63 g/molBCIP is a white to off-white powder that is used as a substrate for visualization of alkaline phosphatase. The end product is insoluble and purple when used with nitroblue tetrazoli..