Advantages (Cat. # PIE25)
- High Yield: >400 µg of pure transfection grade plasmid DNA from 100 ml of cultured bacterial cells
- Efficient endotoxin removal (<0.1 EU/µg DNA) verified by LAL
- Sample Volume: 50-300 ml of cultured bacterial cells
- Format: plasmid midi anion-exchange resin column
- Elution Volume: 500 µl - 2 ml
- Operation time: within 2 hours (only 80 minutes when combined with the Presto™ Plasmid Concentrator)
- Storage: dry at room temperature (15-25ºC)
Applications
Transfection, Sequencing, Ligation, PCR, In-vitro Transcription, Microinjection, Restriction Enzyme Digestion, Gene Gun
Components
- PM1, PM2, PM3 Buffer
- TrueBlue Lysis Buffer
- PER Buffer
- PEQ Buffer
- PW Buffer
- PEL Buffer
- RNase A
- Plasmid Midi Columns
Quality Control
The quality of the Geneaid™ Midi Plasmid Kit (Endotoxin Free) is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Plasmid DNA from a 100 ml overnight E. coli (DH5α) culture, containing plasmid pBluescript (A600 > 2 U/ml) is purified using the Geneaid™ Midi Plasmid Kit. Following the purification process, a yield of more than 400 μg is obtained and the A260/A280 ratio is between 1.8-2.0. The purified plasmid DNA (1 µg) is used in EcoRI digestion, and analyzed by electrophoresis.
Plasmid Midi Kit Endotoxin Free Functional Test Data
Figure 1. Plasmid DNA from a 100 ml overnight E. coli (DH5α) culture, containing plasmid pBluescript (OD600 = 3.65) was purified using the Geneaid™ Midi Plasmid Midi. The purified supercoiled plasmid DNA was used in EcoRI digestion. Plasmid DNA yield was determined by spectrophotometer and analyzed by electrophoresis on a 1% agarose gel. M = Geneaid 1 Kb DNA Ladder
Test | DNA Conc. | A260/280 | A260/230 | Yield |
1 | 211.2 µg/ml | 1.87 | 2.27 | 422.4 µg |
2 | 216.6 µg/ml | 1.87 | 2.27 | 433.2 µg |
3 | 224.8 µg/ml | 1.87 | 2.28 | 449.6 µg |
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Plasmid Purification | |
Endotoxin level | <0.10 EU/µg |
Prep Size | Midi |
Technology | Anion Exchange Resin |
DNA/RNA Purification | |
Yeld up to | 800µg |
- Model PIE25
- Weight: 2.50kg
- Dimensions (L x W x H): 20.00cm x 25.00cm x 20.00cm