High-Speed Plasmid Advance Mini Kit (100 rxns)

Advantages (Cat. # PD100, PD300)

• Purify plasmid DNA or cosmid DNA within 15 minutes!
• High Yield: up to 30 μg of pure plasmid DNA or cosmid DNA 
• Plasmid Size: 1-15 kb
• Sample Volume: 1-5 ml of bacterial cells
• Spin Columns: glass fiber membrane optimized for plasmid DNA purification
• Elution Volume: 30-100 µl
• Storage: dry at room temperature (15-25ºC)

Applications

Restriction Enzyme Digestion, Library Screening, Ligation, PCR, Transformation and Sequencing Reactions

Components

• PD1, PD2, PD3 Buffers
• W1 Buffer
• Wash Buffer
• Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C)
• RNase A
• PD Columns
• 2 ml Collection Tubes

Quality Control

The quality of the High-Speed Plasmid Mini Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Plasmid DNA from a 4 ml overnight E. coli (DH5α) culture, containing plasmid pBluescript (A600 > 2 U/ml) is purified using the High-Speed Plasmid Mini Kit. Following the purification process, a yield of more than 20 μg is obtained and the A260/A280 ratio is between 1.8-2.0. The purified plasmid (1 µg) is used in EcoRI digestion, and checked by electrophoresis.

Download User Manual