The Geneaid™ Maxi Plasmid Kit Endotoxin Free was designed for plasmid DNA purification and endotoxin removal using pre-packed anion-exchange resin maxiprep columns to purify plasmid DNA from 200-1600 ml of cultured bacterial cells. TrueBlue Lysis Buffer (an optional color indicator) is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization.This endotoxin free plasmid kit uses a modified alkaline lysis method and RNase treatment to obtain clear cell lysate with minimal genomic DNA/RNA contaminants. Using an efficient gravity-flow procedure, plasmid DNA in the crude lysate is bound to the column and the contaminants are removed with the PW Buffer. The purified plasmid DNA is eluted by a high salt buffer and then precipitated with Isopropanol for desalting. The entire procedure can be completed in 2.5 hours without ultracentrifuges, HPLC or other toxic reagents and the purified plasmid DNA is suitable for Transfection, Sequencing Reactions, Ligation, PCR, and In-vitro Transcription, Microinjection, Restriction Enzyme Digestion and Gene Gun.
Advantages (Cat. # PME10, PME25)
- High Yield: 1.5 mg of pure transfection grade plasmid DNA from 400 ml cultured bacterial cells
- Efficient endotoxin removal (<0.1 EU/µg DNA) verified by LAL
- Large Sample Volume: 200-1600 ml of cultured bacterial cells
- Format: plasmid maxiprep anion-exchange resin column
- Elution Volume: 500 µl - 2 ml
- Operation time: within 2.5 hours
- Storage: dry at room temperature (15-25ºC)
Applications
Transfection, Sequencing, Ligation, PCR, In-vitro Transcription Microinjection, Restriction Enzyme Digestion, Gene Gun
Compnents
- PM1, PM2, PM3 Buffer
- TrueBlue Lysis Buffer
- PER Buffer
- PEQ Buffer
- PW Buffer
- PEL Buffer
- RNase A
- Plasmid Maxi Columns
Quality Control
The quality of the Geneaid™ Maxi Plasmid Kit (Endotoxin Free) is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Plasmid DNA from a 400 ml overnight E. coli (DH5α) culture, containing plasmid pBluescript (A600 > 2 U/ml) is purified using the Geneaid™ Maxi Plasmid Kit. Following the purification process, a yield of more than 900 μg is obtained and the A260/A280 ratio is between 1.8-2.0. The purified plasmid (1 µg) is used in EcoRI digestion, and analyzed by electrophoresis.
Plasmid Maxi Kit Endotoxin Free Funtional Test Data
Figure 1. Plasmid DNA was extracted using the Geneaid™ Maxi Plasmid Kit (Endotoxin Free). The purified supercoiled plasmid DNA [400 ml overnight E. coli (DH5α) culture, containing a 3 kb plasmid pBluescript (A600 > 2 U/ml, OD600 = 4.0)], was used in EcoRI digestion and analyzed by electrophoresis on a 1% agarose gel. M = Geneaid 1 Kb DNA Ladder
| Sample | DNA Conc. | 260/280 | 260/230 | Total Yield |
| Test 1 | 1550.10 µg/ml | 1.87 | 2.25 | 1.55 mg |
| Test 2 | 1540.10 µg/ml | 1.87 | 2.29 | 1.54 mg |
Download User Manual
- Availability: In Stock
- Model PME02
- Weight: 2.50kg
- Dimensions (L x W x H): 20.00cm x 25.00cm x 20.00cm
