The Genomic
DNA Mini Kit (Tissue) is a genomicDNA Extraction Kit optimized
for genomic, mitochondrial and virus DNA purification from a variety of animal
tissue and insects. This genomic DNA extraction kit includes
Micropestles to ensure efficient homogenization of tissue samples and to
shorten the time in the Lysis Step. Proteinase K and chaotropic salt are used
to lyse cells and degrade protein, allowing DNA to be easily bound by the glass
fiber matrix of the genomic DNA spin column. Once any contaminants have been
removed, using a Wash Buffer (containing ethanol), the purified DNA is eluted
by a low salt Elution Buffer, TE or water and is ready for use in a variety of
downstream applications such as PCR, AFLP/PADP, RFLP, Southern Blotting,
Real-time PCR. For RNA extraction from tissue samples, the Total RNA Mini
Kit (Tissue) is also available.
Advantages (Cat. # GT050, GT100, GT300)
- Purify
genomic DNA from tissue samples in 30 minutes!
High Yield: 10-20 µg (0.5
cm mouse tail), 20-50 µg (20 mg mouse kidney)
- Sample
Size: up to 30 mg of tissue (tailsnips, liver, kidney,
brain, earpunches, insects etc.)
- Spin Columns: glass fiber membrane
optimized for genomic DNA extraction
- Elution Volume: 30-100 µl
- Provided Micropestles are certified
DNase and RNase-free
- Storage: dry
at room temperature (15-25°C)
Applications
PCR, AFLP/PADP, RFLP, Southern Blotting, Real-time PCR
Components
- Micropestle
- GT
Buffer
- GBT
Buffer
- W1
Buffer
- Wash
Buffer
- Elution
Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
- Proteinase
K
- GS
Columns
- 2 ml
Collection Tubes
Quality Control
The quality of the
Genomic DNA Mini Kit (Tissue) is tested on a lot-to-lot basis according to
Geneaid's ISO-certified quality management system. Genomic DNA
from a 20 mg mouse liver sample is extracted using the Genomic DNA
Mini Kit (Tissue). The purified DNA (10 μg with an A260/A280 ratio of 1.8-2.0)
is quantified with a spectrophotometer and checked by
electrophoresis.
- Model GT050
- Weight: 0.00kg