The qScript cDNA Synthesis Kit is a sensitive and easy-to-use solution for RNA quantification using two step RT-qPCR. The novel qScript Reaction Mix provides all the necessary components for cDNA synthesis except enzyme and RNA template. qScript reverse transcriptase is a mixture of an engineered MMLV RT and a ribonuclease inhibitor protein. This economical, highly stable stabilize, 2-component reagent system has been rigorously optimized to ensure sensitive and linear RNA detection with a wide-range of input RNA and relative abundance. Reagent performance is unaffected by repetitive freeze/thaw cycling (>20X), conferring greater ease-of-use and assay performance consistency. Oligo (dT) and random primers are pre-blended in a precise ratio to provide equal representation of 5' and 3'-sequences for accurate gene expression quantification. The resulting cDNA product is directly compatible with all qPCR chemistries or conventional end-point RT-PCR of amplified fragments ≤1 kb in length.
Contents
- 5X concentrated master mix containing: titered primer blend (oligo dT(20) and random hexamer), qPCR-optimized dNTP blend and flexible magnesium titration
- 50X concentrated qScript reverse transcriptase
- Nuclease-free water
qScript cDNA Synthesis Kit components are stable for up to 1 year when stored in a constant temperature freezer at or below -20°C. Reagent performance is unaffected by 20+ repetitive freeze-thaw. Thaw completely on ice then pulse vortex to mix and briefly spin-down to collect contents before opening tube. Concentrated reagents are viscous. When drawing out material, touch pipette tip to liquid interface but do not immerse.
Download User Manual
RT-PCR | |
Application | Economical System (2 comp) |
Format | cDNA Kits |
RNA target | ≤ 1kb |
- Availability: In Stock
- Model 95047100
- Weight: 0.05kg
- Dimensions (L x W x H): 1.00cm x 8.00cm x 10.00cm