The qScript One-Step
SYBR Green RT-qPCR Kit is a convenient and highly sensitive solution for
quantitative RT-PCR of RNA templates (RT-qPCR) using SYBR Green I dye detection
and gene-specific primers. cDNA synthesis and PCR amplification are carried out
in the same tube without opening between procedures.The proprietary reaction
buffer has been specifically formulated to maximize activities of both reverse
transcriptase and Taq DNA polymerase while minimizing the potential for
primer-dimer and other non-specific PCR artifacts. This reagent is compatible
with both fast and standard qPCR cycling protocols. Precise amplification is
essential for successful RT-qPCR with SYBR Green I technology since this dye
binds to all dsDNA generated during amplification. This 1-step reagent contains
ultra-pure AccuStart™ hot start Taq DNA polymerase that is completely arrested
prior to the initial PCR denaturation step. Upon heat activation at 95°C, the
antibodies are rapidly and irreversibly denatured, releasing a fully active
high-yielding Taq DNA polymerase mutant.
qRT- PCR | |
Chemistry | TaqMan |
Cycling Protocol | Conventional |
Instrument Compatibility | No ROX |
Multiplexing | No |
Tough Samples | No |
- Availability: In Stock
- Model 95057200
- Weight: 0.05kg
- Dimensions (L x W x H): 1.00cm x 8.00cm x 10.00cm