EvaGreen® dye is a green fluorescent nucleic acid dye with features that make the dye useful for several applications including qPCR and DNA melt curve analysis, real-time monitoring of thermophilic helicase-dependent amplification (tHDA), and capillary gel electrophoresis. The dye is essentially nonfluorescent by itself, but becomes highly fluorescent upon binding to dsDNA. The DNA-bound dye has excitation and emission spectra very close to those of fluorescein (FAM) or SYBR® Green I, making it readily compatible with instruments equipped with the 488 nm argon laser or any visible light excitation with wavelength in the region. EvaGreen® dye is extremely stable both thermally and hydrolytically, providing convenience during routine handling. EvaGreen® dye is nonmutagenic and noncytotoxic because it is impermeable to cell membranes, unlike SYBR® Green I, which enters cell rapidly and is known to be a powerful mutation-enhancer (Ohta, et el. Mutat. Res. 492, 91(2001)).
The unique properties of EvaGreen® dye have made it particularly useful in quantitative real-time PCR (qPCR) applications. Compared with the widely used SYBR® Green I, EvaGreen® dye is generally less inhibitory toward PCR and less likely to cause nonspecific amplification. As a result, EvaGreen® dye can be used at a much higher dye concentration than SYBR® Green I, resulting in a high resolution signal for droplet digital PCR, real-time PCR and melt curve analysis.
EvaGreen dye and applications are covered under US patent nos. 7,803,943 and 7,776,567 and pending international patents.
Features:
- Low PCR inhibition
- Exhibits much less PCR inhibition than SYBR® Green I via a smart “release-on-demand” DNA-binding technology
- Highly Sensitive
- Low PCR inhibition of the dye permits a higher dye concentration to be used for much greater qPCR and melt curve analysis signals
- Nonmutagenic and noncytotoxic
- Nonmutagenic and noncytotoxic by standard Ames test; completely impermeable to cell membranes
- Compatible with fast PCR protocols
- Minimal interference to PCR makes it possible to significantly shorten the chain extension time
- Compatible with multiplex PCR
- No dye migration from amplicon to amplicon when used at the recommended concentration
- Unsurpassed thermal stability, chemical stability and photostability
- No detectable dye decomposition in PCR buffer at 95-100°C for 48 hours; highly stable under either alkaline or acidic condition; withstands repeated freeze-thaw cycles
- Spectrally similar to SYBR® Green I
- Compatible with all major brand qPCR instruments and enzyme systems
The unique properties of EvaGreen® dye have made it particularly useful in quantitative real-time PCR (qPCR) applications. Compared with the widely used SYBR® Green I, EvaGreen® dye is generally less inhibitory toward PCR and less likely to cause nonspecific amplification. As a result, EvaGreen® dye can be used at a much higher dye concentration than SYBR® Green I, resulting in a high resolution signal for droplet digital PCR, real-time PCR and melt curve analysis.
EvaGreen dye and applications are covered under US patent nos. 7,803,943 and 7,776,567 and pending international patents.
Features:
- Low PCR inhibition
- Exhibits much less PCR inhibition than SYBR® Green I via a smart “release-on-demand” DNA-binding technology
- Highly Sensitive
- Low PCR inhibition of the dye permits a higher dye concentration to be used for much greater qPCR and melt curve analysis signals
- Nonmutagenic and noncytotoxic
- Nonmutagenic and noncytotoxic by standard Ames test; completely impermeable to cell membranes
- Compatible with fast PCR protocols
- Minimal interference to PCR makes it possible to significantly shorten the chain extension time
- Compatible with multiplex PCR
- No dye migration from amplicon to amplicon when used at the recommended concentration
- Unsurpassed thermal stability, chemical stability and photostability
- No detectable dye decomposition in PCR buffer at 95-100°C for 48 hours; highly stable under either alkaline or acidic condition; withstands repeated freeze-thaw cycles
- Spectrally similar to SYBR® Green I
- Compatible with all major brand qPCR instruments and enzyme systems
Download Flyer EvaGreen
qPCR | |
Chemistry | EvaGreen |
- Availability: In Stock
- Model 31001
- Weight: 0.05kg
- Dimensions (L x W x H): 1.00cm x 5.00cm x 1.00cm