Bestsellers
2-3 Days
HotStart DNA polymerase (250 units)
HotStart Taq DNA Polymerase is a high purity, recombinant Taq DNA polymerase
preparation with high avidity monoclonal antibodies that bind the polymerase
and keep it inactive prior to the initial PCR denaturation step. Upon heat
activation (1 minute at 94°C ),
the antibodies denature irreversibly, releasing fully active, unmodified Taq
DNA polymerase. This enables specific and efficient primer extension with the
convenience of room temperature reaction assembly. Non-specific extension of
primers at low temperatures is a common cause of artifacts and poor sensitivity
in PCR. The HotStart Polymerase automatic hot-start enables specific and
efficient primer extension in the PCR process with the added convenience of
room temperature reaction assembly. The included 10X PCR Buffer II is a new
optimized buffer that provides higher product yield, improved specificity, and
enhanced multiplexing capability.
Activated HotStart Taq DNA Polymerase possesses 5´->3´DNA polymerase
activity and a double-strand specific 5´->3´exonuclease. The polymerase does
not have 3´-exonuclease activity and is free of any contaminating endo or
exonuclease activities. One unit is defined as the amount of enzyme that will
incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C . HotStart Taq DNA Polymerase
contains extremely low levels of residual host, E. coli genomic
DNA.
Content
HotStart Taq DNA Polymerase 5 units/μL in 50% glycerol, Download User Manual |
PCR | |
Amplicon Size | Up to 5 kb |
HotStart | Yes |
PCR type | HotStart |
$175.45
- Availability: 2-3 Days
- Model PE3009
- Weight: 0.00kg