Menu
Your Cart
Solicite sus oligos online, al mejor precio y tiempo de entrega 48 hs Click here

EvaGreen® dye, in water (1 ml)

EvaGreen® dye, in water (1 ml)
Bestsellers In Stock
EvaGreen® dye, in water (1 ml)

EvaGreen® dye is a green fluorescent nucleic acid dye with features that make the dye useful for several applications including qPCR and DNA melt curve analysis, real-time monitoring of thermophilic helicase-dependent amplification (tHDA), and capillary gel electrophoresis. The dye is essentially nonfluorescent by itself, but becomes highly fluorescent upon binding to dsDNA. The DNA-bound dye has excitation and emission spectra very close to those of fluorescein (FAM) or SYBR® Green I, making it readily compatible with instruments equipped with the 488 nm argon laser or any visible light excitation with wavelength in the region. EvaGreen® dye is extremely stable both thermally and hydrolytically, providing convenience during routine handling. EvaGreen® dye is nonmutagenic and noncytotoxic because it is impermeable to cell membranes, unlike SYBR® Green I, which enters cell rapidly and is known to be a powerful mutation-enhancer (Ohta, et el. Mutat. Res. 492, 91(2001)).

The unique properties of EvaGreen® dye have made it particularly useful in quantitative real-time PCR (qPCR) applications. Compared with the widely used SYBR® Green I, EvaGreen® dye is generally less inhibitory toward PCR and less likely to cause nonspecific amplification. As a result, EvaGreen® dye can be used at a much higher dye concentration than SYBR® Green I, resulting in a high resolution signal for droplet digital PCR, real-time PCR and melt curve analysis.

EvaGreen dye and applications are covered under US patent nos. 7,803,943 and 7,776,567 and pending international patents.

Features:

  1. Low PCR inhibition
  2. Exhibits much less PCR inhibition than SYBR® Green I via a smart “release-on-demand” DNA-binding technology
  3. Highly Sensitive
  4. Low PCR inhibition of the dye permits a higher dye concentration to be used for much greater qPCR and melt curve analysis signals
  5. Nonmutagenic and noncytotoxic
  6. Nonmutagenic and noncytotoxic by standard Ames test; completely impermeable to cell membranes
  7. Compatible with fast PCR protocols
  8. Minimal interference to PCR makes it possible to significantly shorten the chain extension time
  9. Compatible with multiplex PCR
  10. No dye migration from amplicon to amplicon when used at the recommended concentration
  11. Unsurpassed thermal stability, chemical stability and photostability
  12. No detectable dye decomposition in PCR buffer at 95-100°C for 48 hours; highly stable under either alkaline or acidic condition; withstands repeated freeze-thaw cycles
  13. Spectrally similar to SYBR® Green I
  14. Compatible with all major brand qPCR instruments and enzyme systems
DownloadEvaGreen® dye is a green fluorescent nucleic acid dye with features that make the dye useful for several applications including qPCR and DNA melt curve analysis, real-time monitoring of thermophilic helicase-dependent amplification (tHDA), and capillary gel electrophoresis. The dye is essentially nonfluorescent by itself, but becomes highly fluorescent upon binding to dsDNA. The DNA-bound dye has excitation and emission spectra very close to those of fluorescein (FAM) or SYBR® Green I, making it readily compatible with instruments equipped with the 488 nm argon laser or any visible light excitation with wavelength in the region. EvaGreen® dye is extremely stable both thermally and hydrolytically, providing convenience during routine handling. EvaGreen® dye is nonmutagenic and noncytotoxic because it is impermeable to cell membranes, unlike SYBR® Green I, which enters cell rapidly and is known to be a powerful mutation-enhancer (Ohta, et el. Mutat. Res. 492, 91(2001)).

The unique properties of EvaGreen® dye have made it particularly useful in quantitative real-time PCR (qPCR) applications. Compared with the widely used SYBR® Green I, EvaGreen® dye is generally less inhibitory toward PCR and less likely to cause nonspecific amplification. As a result, EvaGreen® dye can be used at a much higher dye concentration than SYBR® Green I, resulting in a high resolution signal for droplet digital PCR, real-time PCR and melt curve analysis.

EvaGreen dye and applications are covered under US patent nos. 7,803,943 and 7,776,567 and pending international patents.

Features:

  1. Low PCR inhibition
  2. Exhibits much less PCR inhibition than SYBR® Green I via a smart “release-on-demand” DNA-binding technology
  3. Highly Sensitive
  4. Low PCR inhibition of the dye permits a higher dye concentration to be used for much greater qPCR and melt curve analysis signals
  5. Nonmutagenic and noncytotoxic
  6. Nonmutagenic and noncytotoxic by standard Ames test; completely impermeable to cell membranes
  7. Compatible with fast PCR protocols
  8. Minimal interference to PCR makes it possible to significantly shorten the chain extension time
  9. Compatible with multiplex PCR
  10. No dye migration from amplicon to amplicon when used at the recommended concentration
  11. Unsurpassed thermal stability, chemical stability and photostability
  12. No detectable dye decomposition in PCR buffer at 95-100°C for 48 hours; highly stable under either alkaline or acidic condition; withstands repeated freeze-thaw cycles
  13. Spectrally similar to SYBR® Green I
  14. Compatible with all major brand qPCR instruments and enzyme systems
Download User Manual

Download Flyer EvaGreen

qPCR
Chemistry EvaGreen

Write a review

$108.00
  • Availability: In Stock
  • Model 31001
  • Weight: 0.05kg
  • Dimensions (L x W x H): 1.00cm x 5.00cm x 1.00cm
Ritips®evolution 5,0 ml (100 u)
Most Viewed
Ritips® evolution are engineered to provide universal syringes in line with the Ripette® pro as well as with most other dosing systems. Ritips® evolut..
$187.00
Ritips®evolution 2,5 ml (100 u)
Most Viewed
Ritips® evolution are engineered to provide universal syringes in line with the Ripette® pro as well as with most other dosing systems. Ritips® evolut..
$187.00
Ritips®evolution 5,0 ml (100 u)
Most Viewed
Ritips® evolution are engineered to provide universal syringes in line with the Ripette® pro as well as with most other dosing systems. Ritips® evolut..
$187.00
Ritips®evolution 1,0 ml (100 u)
Most Viewed
Ritips® evolution are engineered to provide universal syringes in line with the Ripette® pro as well as with most other dosing systems. Ritips® evolut..
$187.00
EvaGreen® dye, in water (1 ml)
Bestsellers In Stock
EvaGreen® dye is a green fluorescent nucleic acid dye with features that make the dye useful for several applications including qPCR and DNA melt curv..
$108.00
1 kb DNA ladder (50 ug)
Bestsellers
Serves as molecular weight standards for agarose gel electrophoresis. Suitable for sizing of PCR products or other double-stranded DNA fragments. Frag..
$63.00
100 bp DNA ladder (50 ug)
Bestsellers
Serves as molecular weight standards for electrophoresis for both agarose and polyacrylamide gels. Suitable for sizing of PCR products or other double..
$85.00
UV/Vis Nano Spectrophotometer UV/Vis Nano Spectrophotometer
Bestsellers Special Offers 2-3 Days
Nabi- UV/Vis Nano Spectrophotometer is a miniature spectrophotometer that can measure cuvette and microvolume samples. Using spectrometer technology, ..
$14,500.00
Assay 1 (RdRRP) 1000 rxns
Bestsellers
RdRP_SARSr-F2 GTGARATGGTCATGTGTGGCGGRdRP_SARSr-R1 CARATGTTAAASACACTATTAGCATARdRP_SARSr-P2 FAM-CAGGTGGAACCTCATCAGGAGATGC-BHQ1(the above ..
$375.00
200uL Racked Filtered Vertex Tips, Clear, Graduated, NoStick® (96 tips/rack)
Bestsellers
Features10uL to 1250uLClean Liquid ReleaseGraduatedFree of DNase, RNase, DNA and PCR inhibitorsFiltered tips feature HDPE barrier that won't contamina..
$45.00
1mL Racked Filtered Vertex Tips, Clear, Graduated, NoStick® (96 tips/rack)
Bestsellers
Features10uL to 1250uLClean Liquid ReleaseGraduatedFree of DNase, RNase, DNA and PCR inhibitorsFiltered tips feature HDPE barrier that won't contamina..
$35.00
Biometra TOne Thermal Cycler Biometra TOne Thermal Cycler
Bestsellers Special Offers In Stock
Biometra TOne - Optimal Amplification Performance1.       Optimal price – performance ratio2.    &n..
$7,900.00
UV/Vis Nano Spectrophotometer UV/Vis Nano Spectrophotometer
Bestsellers Special Offers 2-3 Days
Nabi- UV/Vis Nano Spectrophotometer is a miniature spectrophotometer that can measure cuvette and microvolume samples. Using spectrometer technology, ..
$14,500.00