pHA-CMV-TOPO Vector is protein expression vector which has CMV promoter that leads expression gene linked to HA peptide at N-terminal and has ampicillin antibiotic marker. It is transient mammalian expression vector which can produce high concentrate protein (0.1mg/L ~ 1mg/L). pHA-CMV-TOPO Vector is treated with topoisomerase (topoisomerase is conjugated to each end of vector). So, ligation reaction is performed without ligase in a short time and high yield of cloning result can be expected.
Applications
- Cloning and expression of Taq polymeraseamplified genes in mammalian cells
- Detection of gene expression using anti-HA antibody
Contents
- pHA-CMV-TOPO Vector
- Salt Solution
- CMV-F Primer
- CMV-24R Primer
Standard reaction procedure
∙ PCR reaction
∙ Mix pHA-CMV-TOPO Vector with PCR products
- PCR Product0.5~4 ㎕
- Salt Solution 1 ㎕
- Sterile Water up to 5 ㎕
- pFlag-CMV-TOPO Vector 1 ㎕
- Final Volume 6 ㎕
∙ Incubation at room temperature for 5min
∙ Transformation into E.coli
∙ Colony analysis
∙ Analysis of Cloned gene: orientation, open reading frame and sequence
- Availability: In Stock
- Model DR02205
- Weight: 0.00kg