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Bst DNA Polymerase, large fragment (8000 units)

Bst DNA Polymerase, large fragment (8000 units)
Bst DNA Polymerase, large fragment (8000 units)
Bestsellers In Stock
Bst DNA Polymerase, large fragment (8000 units)
Bst DNA Polymerase, large fragment (8000 units)
Bst DNA Polymerase, large fragment (8000 units)

Description:
A recombinant E. coli strain carrying the BST DNA Polymerase gene (large fragment).

Application:

- Sequencing through problematic secondary structures

Source:
Bst DNA Polymerase (large fragment) is a portion of Bacillus stearothermophilus DNA Polymerase protein that contains 5´-> 3´ polymerase activity, but lacks 5'->3' and 3'->5' exonuclease activities.

Specific Activity: 120,000 U/mg

Supplied in:
10 mM Tris-HCl
50 mM KCl
1.0 mM DTT
0.1 mM EDTA
0.1% Triton X-100
50% Glycerol
pH 7.5 @ 25°C

Supplied with:
10X PCR buffer II

10x PCR Buffer ll: 
200 mM Tris-HCl
100 mM Ammonium Sulfate
100 mM KCl
20 mM MgSO4
1.0% Triton X-100
pH 8.8 @ 25°C

Unit Definition:
1 unit is defined as the amount of polymerase required to convert 10 nmol of dNTPs into acid insoluble material in 30 minutes at 65°C.

Recommended Storage Condition: -20°C

Reference:
Kiefer, et al. Structure 15 January 1997. 5, 95-108.

Download User Manual

PCR
Amplicon Size -
PCR type Isothermal amplification

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$369.00
  • Availability: In Stock
  • Model BPL-100
  • Weight: 0.05kg
  • Dimensions (L x W x H): 1.00cm x 10.00cm x 17.00cm
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