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MMLV RT (10000 units)

MMLV RT (10000 units)
Bestsellers In Stock
MMLV RT (10000 units)

Moloney Murine Leukemia Virus (M-MuLV) Reverse Transcriptase is an RNA dependent DNA polymerase. It can synthesize a complementary DNA strand initiating from a primer using either RNA or single-stranded DNA as a template. The absence of RNase H activity enhances the synthesis of long cDNAs and therefore the enzyme is recommended for preparing long cDNAs.

Reaction Buffer

10 x Reaction Buffer (0.2 ml): 50 mM Tris-HCl (pH 8.3 at 25°C), 75 mM KCl, 3 mM MgCl2, 10Mm DTT.

Thermal Inactivation: 70ºC for 10 minutes

Storage Buffer

10mM K-phosphate (pH 7.5), 0.1 mM EDTA, 200mM NaCl, 7mM 2-mercaptoethanol and 50% glycerol.

Unit Definition

One unit is defined as the amount of enzyme that is required to incorporate 1nmol of dTTP into an acid-insoluble material in 10 minutes at  37ºC using poly(rA).oligo (dT)

Assay Conditions:

50mM Tris-HCl (pH 8.3), 6mM  MgCl2, 10mM DTT, 0.4mM  poly(rA).oligo (dT)12-18 and 0.5mM (H3)dTTP in a reaction volume of 50ul.

Application:

·        First strand cDNA synthesis
·        DNA labelling
·        RNA analysis by primer extension

Download User Manual

RT-PCR
Format Reverse Transcriptase

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$110.00
  • Availability: In Stock
  • Model PE4014
  • Weight: 0.00kg
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