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qScript cDNA Synthesis Kit (25 rxns)

qScript cDNA Synthesis Kit (25 rxns)
Bestsellers Pre-Order
qScript cDNA Synthesis Kit (25 rxns)

The qScript cDNA Synthesis Kit is a sensitive and easy-to-use solution for RNA quantification using two step RT-qPCR. The novel qScript Reaction Mix provides all the necessary components for cDNA synthesis except enzyme and RNA template. qScript reverse transcriptase is a mixture of an engineered MMLV RT and a ribonuclease inhibitor protein. This economical, highly stable stabilize, 2-component reagent system has been rigorously optimized to ensure sensitive and linear RNA detection with a wide-range of input RNA and relative abundance. Reagent performance is unaffected by repetitive freeze/thaw cycling (>20X), conferring greater ease-of-use and assay performance consistency. Oligo (dT) and random primers are pre-blended in a precise ratio to provide equal representation of 5' and 3'-sequences for accurate gene expression quantification. The resulting cDNA product is directly compatible with all qPCR chemistries or conventional end-point RT-PCR of amplified fragments ≤1 kb in length.

Contents

  1. 5X concentrated master mix containing: titered primer blend (oligo dT(20) and random hexamer), qPCR-optimized dNTP blend and flexible magnesium titration
  2. 50X concentrated qScript reverse transcriptase
  3. Nuclease-free water

Storage & Handling

qScript cDNA Synthesis Kit components are stable for up to 1 year when stored in a constant temperature freezer at or below -20°C. Reagent performance is unaffected by 20+ repetitive freeze-thaw. Thaw completely on ice then pulse vortex to mix and briefly spin-down to collect contents before opening tube. Concentrated reagents are viscous. When drawing out material, touch pipette tip to liquid interface but do not immerse.

Download User Manual

RT-PCR
Application Economical System (2 comp)
Format cDNA Kits
RNA target ≤ 1kb

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$214.78
  • Availability: Pre-Order
  • Model 95047025
  • Weight: 0.05kg
  • Dimensions (L x W x H): 1.00cm x 8.00cm x 10.00cm
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