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UltraScript II RT (2000 u)

UltraScript II RT (2000 u)
In Stock
UltraScript II RT (2000 u)

Ultra Script II Reverse Transcriptase (RNaseH Minus) is an engineered from Moloney Murine Leukemia Virus RT to reduce RNaseH activity and enhance cDNA yield. RnaUsScript RT is a single polypeptide chain with a molecular weight of approximately 78 kDa, is an RNA-dependent DNA polymerase that synthesizes the complementary cDNA strand from a single-stranded RNA template to which a primer has been annealed (1,2). RnaUsScript RT also efficiently extend   primers hybridized  to single-stranded DNA. This enzyme is expressed in E. coli (2) and purified to free of exogenous RNase activity, a common contaminant in commercial preparations of reverse transcriptase.The enzyme is used to synthesize first-strand cDNA up to 12 kb and 5X cDNA Synthesis buffer is provided.

Contents

  1. • Ultra Script II Reverse Transcriptase                                               10 ml
  2. • 5X cDNA Synthesis Buffer  {250 mM Tris-HCl(pH 8.3 at 25oC),
  3.   375 mM KCl, 15 mM MgCl250 mM DTT, and enhancer}           0.4 ml

Storage Buffer

20 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.01% (v/v) NP40S, 50% (v/v)  glycerol

Download User Manual

RT-PCR
Format Reverse Transcriptase

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$90.00
  • Availability: In Stock
  • Model PE5012
  • Weight: 0.00kg
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