qScript XLT cDNA Supermix (25 rxns)
qScript XLT cDNA SuperMix is a next-generation tool for first-strand cDNA synthesis, providing a highly sensitive and easy-to-use solution for two-step RT-PCR and RT-qPCR. qScript XLT is an engineered M-MLV reverse transcriptase mutant with reduced RNase H activity and improved yield and stability at higher temperatures. Combined with a precise mixture of reaction components, this SuperMix enables superior results over a wide dynamic range of input RNA, with up to 8-fold higher sensitivity than our previous qScript cDNA SuperMix cDNA synthesis kits, which utilize an engineered RNase H(+) reverse transcriptase mutant.
Contents
Single-tube, 5X concentrated reagent containing:
- Reaction buffer with optimized concentrations of molecular-grade MgCl2, dATP, dCTP, dGTP, dTTP
- Recombinant ribonuclease inhibitor protein (RIP)
- qScript XLT reverse transcriptase
- Titrated concentrations of random hexamer and oligo(dT) primer
- Proprietary enzyme stabilizers and performance-enhancing additives
qScript XLT cDNA SuperMix is stable for up to 1 year when stored in a constant temperature freezer at or below -20°C. SuperMix reagent shows no loss in functional performance after 20 cycles of freezing on dry ice and thawing on ice. Thaw completely on ice then pulse vortex to mix and briefly spin-down to collect contents before opening tube. 5X concentrated reagent is viscous. When drawing out material, touch pipette tip to liquid interface but do not immerse.
Download User Manual
RT-PCR | |
Application | RNA template complex |
Format | cDNA Kits |
RNA target | ≤ 1kb |
$353.93
- Availability: In Stock
- Model 95161025
- Weight: 0.05kg
- Dimensions (L x W x H): 1.00cm x 8.00cm x 10.00cm