AccuMelt HRM Supermix, (250 rxns)

AccuMelt HRM SuperMix is a 2x concentrated, ready to use reaction cocktail for detection of genetic variations using high resolution melting (HRM) analysis. It includes all required components except for primers and DNA template. HRM is a closed tube, rapid and cost effective procedure for characterization of sequences differences immediately following PCR amplification. It is based on the melting (dissociation) behavior of a PCR product as it transitions from double stranded to single stranded DNA in the presence of a fluorescent dsDNA binding dye.

Content

• 2 x reaction buffer containing optimized concentrations of MgCl2
• dNTPs (including dUTP)
• AccuStart Taq DNA Polymerase
• SYTO® 9 green-fluorescent dye
• stabilizers
• Free Mg2+ = 0.8mM at 1x final concentration.