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AccuStart Genotyping ToughMix, Rox (250 rxns)

AccuStart Genotyping ToughMix, Rox (250 rxns)
AccuStart Genotyping ToughMix, Rox (250 rxns)

AccuStart Genotyping ToughMix is a 2X concentrated ready-to-use reaction cocktail for PCR amplification of DNA templates that overcomes many known inhibitors of PCR often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It is a versatile and robust real-time qPCR reagent that provides maximum sensitivity and PCR efficiency with a variety of fluorogenic probe chemistries, including TaqMan hydrolysis probes. The only user-supplied components are primers, probe(s), and DNA template. Inert AccuVue plate loading dye is compatible with either white or clear PCR plates and helps to minimize pipette error and provides visual confirmation of thorough mixing.

This proprietary formulation has been rigorously optimized to destabilize probes that differ by only a single base-pair substitution to provide precise allelic descrimination for genotyping single nucleotide polymorphisms (SNPs) with either hydrolysis (TaqMan) or FRET-based fluorescent probes.

A key component of Genotyping ToughMix is an ultra pure, highly processive thermostable DNA polymerase that is combined with high avidity monoclonal antibodies. This proprietary polymerase mix is highly resistant to PCR inhibitors and provides an extremely stringent automatic hot-start allowing reaction assembly, and temporary storage, at room temperature prior to PCR amplification. PerfeCTa qPCR ToughMix delivers exceptional performance with either fast or conventional PCR cycling protocols.

Features & Benefits

Optimized buffer chemistry destabilizes single base-pair mismatch probes, providing superior allelic discrimination and cluster separation.

Consistent and reliable performance with exceptional reagent stability - reagent performance unaffected by 20X repetitive freeze-thaw or 30 days at 22°C.

Easy-to-use 2X concentrated master mixes with AccuVue plate loading dye and optimized passive reference dye for simplified reaction setup.

Superior assay sensitivity with AccuStart II enzyme technology – maximum-yielding, engineered Taq DNA polymerase with ultrapure, stringent antibody hotstart.

Contains proprietary ToughMix buffer technology to withstand PCR inhibition common with clinical specimens and plant tissues.

Supports efficient vortex mixing with proprietary anti-foaming technology.

Rigorously optimized for both fast and conventional thermal cycling parameters.

 Genotyping ToughMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

Content

Single-tube, 2X concentrated reagent containing:

1.      Reaction buffer with optimized concentrations of molecular-grade MgCl2, dATP, dCTP, dGTP, dTTP.
2.      AccuStart II Taq DNA Polymerase.
3.      Inert AccuVue dye.
4.      Proprietary enzyme stabilizers and performance-enhancing additives.
5.      Titrated reference dye (if applicable).

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$435.60
  • Availability: In Stock
  • Model 95116250
  • Weight: 0.05kg
  • Dimensions (L x W x H): 1.00cm x 8.00cm x 10.00cm
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