DNAzol® Direct is a universal reagent for processing biological samples for direct PCR. No isolation of DNA is required. The DNAzol Direct procedure is simple. Lyse a sample in DNAzol Direct for 15 minutes, add an aliquot of the resulting lysate to a PCR mix, and perform amplification of a selected DNA fragment(s). The standard DNAzol Direct procedure supports PCR amplification of DNA fragments up to 8 kb long. DNAzol Direct (U.S. patent 7,727,718) composition and procedure are based on the use of an alkaline solution containing polyethylene glycol and other additives. DNAzol Direct quickly and effectively lyses biological samples, releasing DNA into the lysate. The combined effects of the alkaline pH and chaotropic properties of DNAzol Direct sufficiently inactivate PCR inhibitors including proteases and nucleic acid degradation enzymes. After processing a sample in DNAzol Direct, DNA is denatured into a single-stranded form, RNA is hydrolyzed, and proteins are denatured and partially hydrolyzed. Due to its unique composition, the DNAzol Direct lysate does not require neutralization before its use in PCR. The resulting pH of a PCR mix containing less than 10% of the lysate is within the effective range for PCR.
DNAzol® Direct is designed to process a wide range of samples including animal, plant, fungi, yeast, bacterial, and viral samples. It has been used with blood, serum and plasma, saliva, buccal swabs, blood cards, hairs and feathers, leaves and seeds, and formalin-fixed tissues.
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- Availability: In Stock
- Model DN131-50
- Weight: 0.00kg