TRI Reagent® LS is a complete and ready to use reagent for the isolation of total RNA or the simultaneous isolation of RNA, DNA and proteins from liquid samples of human, animal, plant, yeast, bacterial and viral origin. TRI Reagent® LS is the improved version of the popular single-step method of total RNA isolation (1, 2). This highly reliable technique performs well with small and large sample volumes, and allows simultaneous processing of a large number of samples. TRI Reagent® LS and the single-step method are subjects of the international patents. TRI Reagent® LS combines phenol and guanidine thiocyanate in a mono-phase solution to facilitate the immediate and most effective inhibition of RNase activity. A biological sample is homogenized or lysed in TRI Reagent® LS and the lysate is separated into aqueous and organic phases by bromochloropropane or chloroform addition and centrifugation. RNA remains exclusively in the aqueous phase, DNA in the interphase, and proteins in the organic phase. RNA is precipitated from the aqueous phase by the addition of isopropanol, and the RNA pellet is washed with ethanol and solubilized. DNA and proteins are sequentially precipitated from the interphase and organic phase with ethanol and isopropanol, washed with ethanol and solubilized.
TRI Reagent® LS is used for cell suspensions and other liquid samples. Fifty milliliters is sufficient to process 65 samples of 0.25 ml each.
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- Availability: In Stock
- Model TR120200
- Weight: 0.00kg