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Geneaid™ Maxiprep Plasmid Kit (2 rxns)

Geneaid™ Maxiprep Plasmid Kit (2 rxns)
Geneaid™ Maxiprep Plasmid Kit (2 rxns)
The Geneaid™ Maxiprep Plasmid Kit was designed for plasmid DNA purification using pre-packed anion-exchange resin maxiprep plasmid columns to purify plasmid DNA from 200-1600 ml of cultured bacterial cells. TrueBlue Lysis Buffer (an optional color indicator) is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization. This plasmid kit uses a modified alkaline lysis method and RNase treatment to obtain clear cell lysate with minimal genomic DNA/RNA contaminants. Using an efficient gravity-flow procedure, plasmid DNA in the crude lysate is bound to the plasmid maxi column and the contaminants are removed with PW Buffer. The purified plasmid DNA is eluted by a high salt buffer and then precipitated with Isopropanol for desalting. The entire procedure can be completed in 2.5 hours without ultracentrifuges, HPLC or other toxic reagents and the purified plasmid DNA is suitable for Transfection, Sequencing Reactions, Ligation, PCR, and In-vitro Transcription, Microinjection, Restriction Enzyme Digestion and Gene Gun.

Advantages (Cat. # PM010, PM025)

  • High Yield: 1.5 mg of pure transfection grade plasmid DNA from 400 ml cultured bacterial cells
  • Large Sample Volume: 200-1600 ml of cultured bacterial cells
  • Format: plasmid maxi anion-exchange resin columns
  • Elution Volume: 500 µl - 2 ml
  • Operation time: within 2.5 hours
  • Storage: dry at room temperature (15-25ºC)

Applications

Transfection, Sequencing, Ligation, PCR, In-vitro Transcription Microinjection, Restriction Enzyme Digestion, Gene Gun

Compnents

  • PM1, PM2, PM3 Buffer
  • TrueBlue Lysis Buffer
  • PEQ Buffer
  • PW Buffer
  • PEL Buffer
  • RNase A
  • Plasmid Maxi Columns

Quality Control

The quality of the Geneaid™ Plasmid Maxi Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Plasmid DNA from a 400 ml overnight E. coli (DH5α) culture, containing plasmid pBluescript (A600 > 2 U/ml) is purified using the Geneaid™ Maxi Plasmid Kit. Following the purification process, a yield of more than 900 μg is obtained and the A260/A280 ratio is between 1.8-2.0. The purified plasmid (1 µg) is used in EcoRI digestion, and analyzed by electrophoresis.

Plasmid Maxi Kit Funtional Test Data

Figure 1. Plasmid DNA was extracted using the Geneaid™ Maxi Maxi Kit. The purified supercoiled plasmid DNA [400 ml overnight E. coli (DH5α) culture, containing a 3 kb plasmid pBluescript (A600 > 2 U/ml, OD600 = 4.0)], was used in EcoRI digestion and analyzed by electrophoresis on a 1% agarose gel. M = Geneaid 1 Kb DNA Ladder
Sample
DNA Conc.260/280260/230Total Yield
Test 11550.10 µg/ml1.872.251.55 mg
Test 21540.10 µg/ml1.872.291.54 mg 
Protocol DownloadPlasmid DNA Midi and Maxi Kits Specifications
SpecificationsPresto™ Midi Plasmid KitPresto™ Midi Plasmid Kit Endotoxin FreeGeneaid™ Midi Plasmid KitGeneaid™ Midi Plasmid Kit Endotoxin FreeGeneaid™ Maxi Plasmid KitGeneaid™ Maxi Plasmid Kit Endotoxin Free
Cat. #PIF025PIFE25PI025PIE25PM010/25PME10/25
Format
Anion-Exchange
Gravity Flow
Anion-
Exchange
Gravity Flow
Anion-Exchange
Gravity Flow
Anion-
Exchange
Gravity Flow
Anion-Exchange
Gravity Flow
Anion-
Exchange
Gravity Flow
Binding Capacity500 µg500 µg800 µg800 µg1.5 mg1.5 mg
Filter ColumnYesYesN/AN/AN/AN/A
Endotoxin Level<0.15 EU/µg<0.10 EU/µg<0.15 EU/µg<0.10 EU/µg<0.15 EU/µg<0.10 EU/µg
Culture Input50-150 ml50-150 ml50-300 ml50-300 ml200-1600 ml200-1600 ml
Plasmid Size1-20 kb1-20 kb1-20 kb1-20 kb1-20 kb1-20 kb
Typical Yield200-500 µg200-500 µg200-800 µg200-800 µg1-1.5 mg1-1.5 mg
Elution Volume500 µl-2 ml500 µl-2 ml500 µl-2 ml500 µl-2 ml500 µl-2 ml500 µl-2 ml
Operation Time<80 minutes<80 minutes<120 minutes<120 minutes<180 minutes<180 minutes
Download User Manual
Plasmid Purification
Endotoxin level <0.15 EU/µg
Prep Size Maxi
Technology Anion Exchange Resin
DNA/RNA Purification
Yeld up to 2500µg

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$67.85
  • Availability: In Stock
  • Model PM010
  • Weight: 2.50kg
  • Dimensions (L x W x H): 20.00cm x 25.00cm x 20.00cm
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