Advantages (Cat. # GEB100, GEB01K, GEB01K+)
- Isolate high molecular weight DNA for archiving or sensitive downstream applications
- High yield, High quality DNA (A260/A280 = 1.8-2.0)
- Sample: 300 µl of whole blood, 3 ml of whole blood, 10 ml of whole blood, 10 ml of compromised whole blood
- Easily scalable to satisfy larger blood sample volumes
- Minimal hands on time
- Convenient, simple and gentle reagent DNA precipitation method
- Storage: dry at room temperature (15-25ºC), RNase A should be stored at 4ºC for extended periods
Applications
DNA archiving, PCR, AFLP, RFLP/PADP, Southern Blotting, Real-time PCR
Components
- RBC Lysis Buffer
- Cell Lysis Buffer
- Protein Removal Buffer
- DNA Hydration Buffer (10 mM Tris-HCL, 1 mM EDTA, pH8.0)
- RNase A (10 mg/ml) - not included with GEB01K
Quality Control
The quality of the Geneaid™ DNA Isolation Kit (Blood) is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Genomic DNA is isolated from 300 µl of fresh whole human blood. The isolated DNA (5-15 µg with an A260/A280 ratio of 1.8–2.0) is quantified with a spectrophotometer and analyzed by electrophoresis.
Geneaid™ DNA Isolation Kit (Blood) Functional Test Data
Figure 1. High molecular weight genomic DNA (100-200 kb) was extracted using both the Geneaid™ DNA Isolation Kit (Blood) and the Gentra® Puregene® Blood Kit. Genomic DNA was extracted from 300 µl of whole human blood and resolved in 100 µl of DNA Hydration Buffer. 5 µl of the resolved genomic DNA was analyzed by electrophoresis on a 1% agarose gel.
- Availability: In Stock
- Model GEB100
- Weight: 0.00kg