- 3 μl or 5 μl per loading for clear visualization during
electrophoresis on 15-well or 10-well mini-gel, respectively
- 2~3 μl per well for general Western transfering.
- Apply more for thicker (>1.5 mm) or larger gel.
The BlueRAY Prestained Protein Ladder is a three-color
protein standard with 10 pre-stained proteins covering a wide range molecular
weights for 10 to 180 kDa. Proteins are covalently coupled with a blue
chromophore except for two reference bands (one green and one red band at 25 kDa
and 75 kDa respectively) when separated on SDS-PAGE (Trisglycine buffer). The
BlueRAY Prestained Protein Ladder is designed for monitoring protein separated
during SDS polyacrylamide gel electrophoresis, verification of Western transfer
efficiency on membranes (PVDF, nylon or nitrocellulose) and for approximate
sizing of proteins. The ladder is supplied in gel loading buffer and is ready
to use. Do not heat, dilute, add reducing agent before loading.
Contents
Approximately 0.2~0.4 mg/ml of each protein in buffer (20 mM
Trisphosphate pH 7.5 at 25°C), 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and
15% (v/v) Glycerol).
Quality Control
5 μl of BlueRAY Prestained Protein Ladder resolves 10 bands
in 4-20% SDS-PAGE (Tris-glycine buffer) and after Western blotting to PVDF
membrane.
Storage
Stable for up to 2 weeks at 25°C.
Stable for up to 3 months at 4°C. For long term storage,
store at -20°C.
- Availability: In Stock
- Model PL0350
- Weight: 0.20kg
- Dimensions (L x W x H): 1.00cm x 5.00cm x 10.00cm