- 3 μl or 5 μl per loading for clear visualization during
electrophoresis on 15-well or 10-well mini-gel, respectively.
- 1.5~2.5 μl per well for general Western transferring.
- Apply more for thicker (> 1.5 mm) or larger gel.
The BLUeye Prestained Protein Ladder is a three-color
protein standard with 12 pre-stained proteins covering a wide range molecular
weights from 10 to 245 kDa. Proteins are covalently coupled with a blue
chromophore except for two reference bands (one green and one red band at 25
kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine
buffer).The BLUeye Prestained Protein Ladder is designed for monitoring protein
separation during SDS-polyacrylamide gel electrophoresis, verification of
Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and
for approximating the size of proteins. The ladder is supplied in gel loading
buffer and is ready to use.
Contents
Approximately 0.1~0.4 mg/ml of each protein in the buffer
(20 mM Trisphosphate, pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M
Urea, and 15 % (v/v) Glycerol).
Quality Control
Under suggested conditions, BLUeye Prestained Protein Ladder
resolves 12 major bands in 15% SDS-PAGE (Tris-glycine buffer) and after Western
blotting to nitrocellulose membrane.
Storage
Stable for up to 2 weeks at 25°C.
Stable for up to 3 months at 4°C. For long term storage,
store at -20°C.
- Availability: In Stock
- Model PL0450
- Weight: 0.20kg
- Dimensions (L x W x H): 1.00cm x 5.00cm x 10.00cm