The Unveil Unstained Protein Ladder is a mixture of 12
unstained recombinant proteins covering a wide range of molecular weights from
10 to 200 kDa. The proteins resolve into clearly identifiable bands when
separated on the SDS-PAGE gel (MES buffer), with the intensified 25kDa and
85kDa protein bands serving as the two reference bands. The Unveil Unstained Protein Ladder is
designed for monitoring protein separation during SDS-polyacrylamide gel
electrophoresis and verifying Western transfer efficiency on membranes (PVDF,
nylon, or nitrocellulose) and for approximating the size of proteins. The
ladder is supplied in the gel loading buffer and is ready to use.
Storage Buffer
20 mM Tris-phosphate(pH 7.5 at 25°C), 2 % SDS, 0.2 mM
Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol.
Quality Control
Under suggested conditions, Unveil Unstained Protein Ladder
resolves 12 major bands in 4-12% Bis- Tris Gel(MES buffer) and after Western
blotting to the nitrocellulose membrane.
Storage
Stable for up to 2 weeks at 25°C. Stable for up to 3 months
at 4°C.
For long term storage, store at -20°C
- Availability: In Stock
- Model PL1025
- Weight: 0.20kg
- Dimensions (L x W x H): 1.00cm x 5.00cm x 10.00cm