- 3 μl or 5 μl per loading for clear visualization during
electrophoresis on 15-well or 10-well mini-gel, respectively.
- 2.5 μl per well for general Western transferring.
The BLUltra Prestained Protein Ladder is a three-color
protein standard with 10 pre-stained proteins covering a wide range of
molecular weights from 6.5 to 270 kDa. Proteins are covalently coupled with a
blue chromophore except for three reference bands (two orange bands at 30 kDa
and 270 kDa and one green band at 52 kDa) when separated on SDS-PAGE
(Tris-glycine buffer). The BLUltra Prestained Protein Ladder is designed for
monitoring protein separation during SDS-polyacrylamide gel electrophoresis and
verifying Western transfer efficiency on membranes (PVDF, nylon, or
nitrocellulose) and for approximating the size of proteins. The ladder is
supplied in the gel loading buffer and is ready to use.
Storage Buffer
62.5 mM Tris-H3PO4 (pH 7.5 at 25 °C), 1 mM EDTA, 2% SDS, 10
mM DTT, 1 mM NaN3, 33% glycerol.
Quality Control
Under suggested conditions, BLUltra Prestained Protein
Ladder resolves 10 major bands in 4-12% Bis-Tris Gel (MES buffer) and after
Western blotting to the nitrocellulose membrane.
Storage
Stable for up to 2 weeks at 25°C.
Stable for up to 3 months at 4°C.
For long term storage, store at -20°C.
- Availability: In Stock
- Model PL0150
- Weight: 0.20kg
- Dimensions (L x W x H): 1.00cm x 5.00cm x 10.00cm