BDX64 Buffer (2 x 1.25 ml)
MCLAB's BDX64 is a BigDye® enhancing buffer, it is used for DNA sequencing. BDX64 buffer has the same ion strength as the BigDye® premix 3.1 and 1.1.
Up to 64 (0.12 µl bigdye in 10µl reaction) or more fold dilutions by combining with the 5x dilution buffer
Enhances the polymerase activity and reduces the extension time from 4 to 1 minute.
Reduces the signal decline rate and results in even peak distributions.
Optimizes for using with BigDye® Chemistry (ABI) on 310, 3100, 3130/3130xl & 3730/3730xl.
Application:
Component | Volume | Volume |
BigDye® 3.1 | 0.25 µl | 0.5 µl |
5x buffer (cat# SBUF-100) | 0.875 µl | 0.75 µl |
BDX64 buffer | 2 µl | 2 µl |
Template | 100 ng | 100 ng |
Primer | 3.2 pmol | 3.2 pmol |
Add H2O to 10 µl |
Sequencing Examples:
1. Dilution fold 32X:
2. Dilution fold 64X:
3. Dilution fold 128X:
4. Dilution fold 256X:
Signal Distributions: before and after BDX64 was added
Recommended Cycle Conditions:
96°C, 3 minutes. + 30x (96°C, 10 seconds. 50°C, 5 seconds. 60°C, 2 minutes.)
Recommended Storage Condition: -20°C
$504.00
- Availability: In Stock
- Model BDX-100
- Weight: 0.02kg
- Dimensions (L x W x H): 3.00cm x 3.00cm x 6.00cm
-60x60w.png)
