Molecular Formula: CH4N2O
Molecular Weight: 60.06
CAS: 57-13-6
Melting Point: 132.7 °C1
Synonyms: carbamide, carbonyldiamide1
This product is designated as Molecular Biology grade
and is suitable for molecular biology applications. It
has been analyzed for the absence of nucleases and
proteases.
Urea is a prinicipal protein metabolite and the major
product for the removal of free ammonia (NH4
+
)
in vivo. In the urea cycle, the nitrogen atoms that are
incorporated into urea are derived from the amino
group of asparate and from free ammonia. The initial
biosynthesis of urea occurs outside of the urea cycle,
with the condensation of ammonia ion and bicarbonate
to give carbamoyl phosphate. Carbamoyl phosphate
then enters the urea cycle and undergoes successive
enzymatic conversion to citrulline, argininosuccinate,
fumarate, and arginine. Arginine is then hydrolyzed by
arginase to urea and ornithine. Urea is commonly utilized in biochemistry and
molecular biology in various applications. The use of
urea in protocols for denaturing fusion proteins,
solubilization of inclusion bodies, and denaturing
polyacrylamide gel electrophoresis has been
described. In protein electrophoresis, urea helps in
protein denaturation and solubilization, although care
must be exercised because of the potential for urea to
carbamoylate proteins.
- Availability: In Stock
- Model RU2610
- Weight: 0.50kg
- Dimensions (L x W x H): 7.00cm x 7.00cm x 18.00cm