TBE 10 X (500 ml)

Dilution of the TBE stock concentrates to a 1× TBE running buffer results in a buffer containing 89 mM Tris-borate and 2 mM EDTA, pH 8.3. The 5× or 10× stocks may also be added to an acrylamide/bis-acrylamide stock solution for making the PAGE gel. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.

Ready for use in gel electrophoresis after dilution to working concentrations.

Tris-Borate-EDTA buffer has been used for pulsed-field gel electrophoresis (PFGE)