PlasmoTest™ - Mycoplasma Detection Kit (250 samples)
PlasmoTest™ provides a simple, rapid and reliable assay for the visual detection of mycoplasma contamination in cell cultures. This assay is the first to utilize cells to signal the presence of mycoplasma.
PlasmoTest™ features two major constituents: the Mycoplasma sensor cells and the HEK-Blue™ Detection medium.
The Mycoplasma sensor cells detect the presence of mycoplasmas leading to a color change of the HEK-Blue™ Detection medium. The Mycoplasma sensor cells recognize mycoplasmas through Toll-like Receptor 2 (TLR2), a pathogen recognition receptor. In the presence of mycoplasmas, TLR2 initiates a signaling cascade leading to the activation of NF-kB and other transcription factors. These transcription factors induce the secretion of SEAP (secreted embryonic alkaline phosphatase) in the supernatant which is readily detected by the purple/blue coloration of the HEK-Blue™ Detection medium.
• Requires only basic cell culture knowledge
• Hands-on time less than 1 hour. Gives results after overnight incubation
• Detects all cell culture contaminants
• Detects 5.102-5.105 cfu/ml mycoplasmas
• No false positive: a positive result indicates the presence of a cell culture contaminant
$1,320.00
- Availability: In Stock
- Model rep-pt1
- Weight: 0.00kg
