Menu
Your Cart
Solicite sus oligos online, al mejor precio y tiempo de entrega 48 hs Click here

Phytohemagglutinin-M (PHA-M), Liquid

Phytohemagglutinin-M (PHA-M), Liquid
Bestsellers In Stock
Phytohemagglutinin-M (PHA-M), Liquid

Phytohaemagglutinin-M (PHA-M) is used for the stimulation of cell proliferation in lymphocyte culture. Phytohaemagglutinin (or Phytohemagglutinin) is a lectin extracted from red kidney beans (Phaseolus vulgaris). The protein consists of two molecular species, a leucoagglutinin (PHA-L) and an erythroagglutinin (PHA-E). Each of the proteins contains a family of five isolectins, each being a tetramer held together by noncovalent forces. PHA-M is the mucoprotein form and is a crude extract used for the stimulation of cell proliferation in lymphocyte culture. PHA-M also has a powerful erythroagglutinating property and it was originally used for separating leukocytes from whole blood. Working Concentration: Each ml will contain 5mg of protein

Procedure

1. Add 2-4ml of PHA-M (Cat. No. 12-009-1H) per 100ml Karyotyping Medium (Cat. No. 01-198-1).

2. Inoculate approximately 0.5ml of heparinized whole blood into a glass or plastic tube with 10ml of medium (or 106 viable cells per ml).

3. Incubate the culture for 72 hours.

4. Add 0.1-0.2ml of Colcemid Solution (Cat. No. 12-004- 1) to each culture tube. Incubate the culture for an additional 15-30 minutes.

5. Transfer the culture to a centrifuge tube and spin at 500g for 5 minutes.

6. Remove the supernatant and re-suspend the cells in 5-10ml of hypotonic 0.075M KCl (Cat. No. 12-005-1). Incubate at 37ºC for 10-12 minutes.

7. Spin at 500g for 5 minutes.

8. Remove the supernatant, agitate the cellular sediment and add drop-by-drop 5-10ml of fresh, ice-cold fixative made up of 1 part acetic acid to 3 parts methanol. Leave in 4ºC for 10 minutes.

9. Repeat steps 7 and 8.

10. Spin at 500g for 5 minutes.

11. Re-suspend the cell pellet in a small volume 0.5-1ml of fresh fixative, drop onto a clean slide and allow to air dry.

12. At this stage, the preparation can be stained with Orecin or Giemsa. Giemsa banding has become the most widely used technique. The most common method to obtain this staining is to treat slides with Trypsin-EDTA 10X (Cat. No. 03-051-5).

Write a review

$48.40
  • Availability: In Stock
  • Model 12-009-1H
  • Weight: 0.00kg
Ritips®evolution 5,0 ml (100 u)
Most Viewed
Ritips® evolution are engineered to provide universal syringes in line with the Ripette® pro as well as with most other dosing systems. Ritips® evolut..
$187.00
Ritips®evolution 2,5 ml (100 u)
Most Viewed
Ritips® evolution are engineered to provide universal syringes in line with the Ripette® pro as well as with most other dosing systems. Ritips® evolut..
$187.00
Ritips®evolution 5,0 ml (100 u)
Most Viewed
Ritips® evolution are engineered to provide universal syringes in line with the Ripette® pro as well as with most other dosing systems. Ritips® evolut..
$187.00
Ritips®evolution 1,0 ml (100 u)
Most Viewed
Ritips® evolution are engineered to provide universal syringes in line with the Ripette® pro as well as with most other dosing systems. Ritips® evolut..
$187.00
Phytohemagglutinin-M (PHA-M), Liquid
Bestsellers In Stock
Phytohaemagglutinin-M (PHA-M) is used for the stimulation of cell proliferation in lymphocyte culture. Phytohaemagglutinin (or Phytohemagglutinin) is ..
$48.40
1 kb DNA ladder (50 ug)
Bestsellers
Serves as molecular weight standards for agarose gel electrophoresis. Suitable for sizing of PCR products or other double-stranded DNA fragments. Frag..
$63.00
100 bp DNA ladder (50 ug)
Bestsellers
Serves as molecular weight standards for electrophoresis for both agarose and polyacrylamide gels. Suitable for sizing of PCR products or other double..
$85.00
UV/Vis Nano Spectrophotometer UV/Vis Nano Spectrophotometer
Bestsellers Special Offers 2-3 Days
Nabi- UV/Vis Nano Spectrophotometer is a miniature spectrophotometer that can measure cuvette and microvolume samples. Using spectrometer technology, ..
$14,500.00
Assay 1 (RdRRP) 1000 rxns
Bestsellers
RdRP_SARSr-F2 GTGARATGGTCATGTGTGGCGGRdRP_SARSr-R1 CARATGTTAAASACACTATTAGCATARdRP_SARSr-P2 FAM-CAGGTGGAACCTCATCAGGAGATGC-BHQ1(the above ..
$375.00
200uL Racked Filtered Vertex Tips, Clear, Graduated, NoStick® (96 tips/rack)
Bestsellers
Features10uL to 1250uLClean Liquid ReleaseGraduatedFree of DNase, RNase, DNA and PCR inhibitorsFiltered tips feature HDPE barrier that won't contamina..
$45.00
1mL Racked Filtered Vertex Tips, Clear, Graduated, NoStick® (96 tips/rack)
Bestsellers
Features10uL to 1250uLClean Liquid ReleaseGraduatedFree of DNase, RNase, DNA and PCR inhibitorsFiltered tips feature HDPE barrier that won't contamina..
$35.00
Biometra TOne Thermal Cycler Biometra TOne Thermal Cycler
Bestsellers Special Offers In Stock
Biometra TOne - Optimal Amplification Performance1.       Optimal price – performance ratio2.    &n..
$7,900.00
UV/Vis Nano Spectrophotometer UV/Vis Nano Spectrophotometer
Bestsellers Special Offers 2-3 Days
Nabi- UV/Vis Nano Spectrophotometer is a miniature spectrophotometer that can measure cuvette and microvolume samples. Using spectrometer technology, ..
$14,500.00