MEM for suspension cultures, without L-Glutamine (500 ml)

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. 
This MEM is modified as follows:

With

• Glucose
• Magnesium
• Earle's Salts
• Sodium Bicarbonate
• Phenol Red

Without:

• L-Glutamine




This preparation is without L-Glutamine. Add 292.0 mg/L L-Glutamine.
Components	Concentration (mg/L)
INORGANIC SALTS:
Potassium Chloride (KCl)	400.00
Magnesium Sulfate (MgSO4)	97.67
Sodium Chloride (NaCl)	6800.00
Sodium Bicarbonate (NaHCO3)	2200.00
Sodium Phosphate-H2O (NaH2PO4-H2O)	1400.00
OTHER COMPONENTS:
D-Glucose	1000.00
Phenol Red	10.00
AMINO ACIDS:
L-Arginine-HCl	126.00
L-Cystine	24.00
L-Histidine-HCl-H2O	42.00
L-Isoleucine	52.00
L-Leucine	52.00
L-Lysine-HCl	72.50
L-Methionine	15.00
L-Phenylalanine	32.00
L-Threonine	48.00
L-Tryptophan	10.00
L-Tyrosine	36.00
L-Valine	46.00
VITAMINS:
D-Sodium Pantothenate	0.506
Choline Chloride	1.00
Folic Acid	1.00
i-Inositol	2.00
Niacinamide	1.00
Pyridoxal HCl	1.00
Riboflavin	0.10
Thiamine HCl	1.00