MEM-E 10X Conc., Earle's Salts Base, without L-Glutamine, without Sodium Bicarbonate (500 ml)

MEM-Eagle is a modified form of MEM (Minimum Essential Medium). MEM is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, fibroblasts, and primary rat astrocytes. Biological Industries offers a variety of MEM modifications for a wide range of cell culture applications. 

This MEM is modified as follows:

With

• Earle’s Salts
• Phenol Red

Without:

• L-Glutamine
• Sodium Bicarbonate

• Components	Concentration (mg/L)
  INORGANIC SALTS
  Calcium Chloride (CaCl2) (Anhyd.)	2000.0
  Potassium Chloride (KCl)	4000.0
  Magnesium Sulfate (MgSO4)	976.7
  Sodium Chloride (NaCl)	68000.0
  Sodium Bicarbonate (NaHCO3)	22000.0
  Sodium Phosphate-H2O (NaH2PO4-H2O)	1400.0
  OTHER COMPONENTS:
  D-Glucose	10000.0
  Phenol Red	100.0
  AMINO ACIDS:
  L-Arginine-HCl	1260.0
  L-Cystine	240.0
  L-Histidine-HCl-H2O	420.0
  L-Isoleucine	520.0
  L-Leucine	520.0
  L-Lysine-HCl	725.0
  L-Methionine	150.0
  L-Phenylalanine	320.0
  L-Threonine	480.0
  L-Tryptophan	100.0
  L-Tyrosine	360.0
  L-Valine	460.0
  VITAMINS:
  D-Ca Pantothenate	10.0
  Choline Chloride	10.0
  Folic Acid	10.0
  i-Inositol	20.0
  Niacinamide	10.0
  Pyridoxal HCl	10.0
  Riboflavin	1.0
  Thiamine HCl	10.0