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DMEM: Nutrient Mixture F-12 (Ham's) (1:1) without L-Glutamine, with Sodium Bicarbonate, with Hepes 15mM, with Sodium (500 ml)

DMEM: Nutrient Mixture F-12 (Ham's) (1:1) without L-Glutamine, with Sodium Bicarbonate, with Hepes 15mM, with Sodium (500 ml)
DMEM: Nutrient Mixture F-12 (Ham's) (1:1) without L-Glutamine, with Sodium Bicarbonate, with Hepes 15mM, with Sodium (500 ml)

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and fibroblasts. 
This DMEM/F-12 ismodified as follows:

With

  • Sodium Bicarbonate
  • Sodium Pyruvate
  • HEPES

Without:

  • L-Glutamine
Components Concentration (mg/L)
INORGANIC SALTS
Calcium chloride (CaCl2) 116.70
Cupric sulfate (CuSO4-5H2O) 0.0013
Ferric nitrate (Fe(NO3)3-9H20) 0.05
Ferrous sulfate (FeSO4-7H2O) 0.417
Potassium chloride (KCl) 311.80
Magnesium chloride (MgCl2) 28.64
Magnesium sulfate (MgSO4) 48.84
Sodium chloride (NaCl) 6995.50
Sodium bicarbonate (NaHCO3) 1200
Sodium phosphate, mono. (NaH2PO4-H20) 62.50
Sodium phosphate, dibas (Na2HPO4) 71.02
Zinc sulfate (ZnSO4-7H2O) 0.432
OTHER COMPOUNDS
D-Glucose 3151.00
Hypoxanthine 2.05
Linoleic Acid 0.042
Lipoic Acid 0.105
Phenol red 8.10
Putrescine-2HCl 0.081
Sodium Pyruvate 55.00
HEPES 3575.00
Thymidine 0.365
AMINO ACIDS
L-Alanine 4.45
L-Arginine hydrochloride 147.50
L-Asparagine-H2O 7.50
L-Aspartic acid 6.65
L-Cysteine-HCl-H2O 17.56
L-Cystine 24.00
L-Glutamic acid 7.35
Glycine 18.75
L-Histidine-HCl-H2O 31.48
MEM
Format Liquid
Glutamine No glutamine
Phenol Red Yes

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$57.90
  • Availability: In Stock
  • Model 01-170-1A
  • Weight: 0.00kg
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