Description
Broad Transfection Spectrum for Mammalian Cell Types
Cell Lines | Transfection Efficiency (% GFP) | Cell Lines | Transfection Efficiency (% GFP) |
3LL | 51% | K562 | 22% |
Comparisons of Transfection Efficiency and Cytotoxicity of GenJet™ DNA In Vitro Transfection Reagent with the Leading Brand Name Products
Exceptional transfection efficiency of extremely long DNA by GenJet™ DNA In Vitro Transfection Reagent. HEK293 cells were transfected with GFP tagged DNA (total 14 kb) by GenJet™ (upper panel) and FuGene™ 6 (lower panel) respectively. The cells were visualized by Nikon Eclipse Fluorescence microscope with DIC phase (left panel) and FITC (right panel) imaging 24 hours post transfection
Exceptional high titers of lentivirus generated from 293F cells. Three DNAs co-transfected with GenJet™ In Vitro DNA Transfection Reagent (left panel) in comparison of LipoFectamine 2000™ (L2K, right panel). 100 microliter virus supernatant from 293F cells were used to counter-infected 293F cells which was then passed FACS. The numbers at the upper right corner indicate the percentage of transduced cells. The titers of lentivirus generated with GenJet™ and L2K were quantified to be 7.9x106 and 3.1x106 tu/ml respectively
Comparison of cytotoxicity of GenJet™ DNA In Vitro Transfection Reagent with L2K™ on primary murine skin fibroblast. The primary murine fibroblast was incubated with the indicated transfection reagents/pEGFP-C1 (DNA) complexes above for 4 hours in serum-free DMEM High Glucose medium followed by replacement of complete serum-containing medium. The cells were visualized by Nikon Eclipse Fluorescence microscope with DIC phase imaging 24 hours post transfection
- Availability: In Stock
- Model SL100488
- Weight: 0.00kg