The High-Speed Plasmid Advance Kit was designed for plasmid
and cosmid DNA purification from 50-100 ml of cultured bacterial cells using a
plasmid midiprep spin column system. This plasmid kit uses a modified alkaline
lysis method and RNase treatment to obtain clear cell lysate with minimal
genomic DNA/RNA contaminants. In the presence of chaotropic salt, plasmid DNA
in the lysate binds to the glass fiber matrix of the plasmid spin column.
Contaminants are removed with a Wash Buffer (containing ethanol) and the
purified plasmid DNA is eluted by a low salt Elution Buffer, TE or water.
Typical yields are 200-350 μg for high-copy number plasmid or 30-100 μg for
low-copy number plasmid from 50 ml of cultured bacterial cells. DNA phenol
extraction or alcohol precipitation is not required and the entire procedure
can be completed within 35 minutes.
Advantages (Cat. # PA025)
• Purify
plasmid DNA from large volume samples within 35 minutes!
• Spin
column format
• High
Yield: up to 500 μg of plasmid DNA
• Large
Sample Volume: 50-100 ml of cultured bacterial cells
• Glass
fiber membrane optimized for plasmid DNA purification from large volume
bacteria samples
• Elution
Volume: 2 ml
• Storage:
dry at room temperature (15-25ºC)
Applications
Restriction Enzyme Digestion, Library Screening, Ligation,
PCR, Transformation and Sequencing Reactions
Components
• PD1, PD2,
PD3 Buffers
• W1 Buffer
• Wash
Buffer
• Elution
Buffer (10 mM Tris-HCl, pH8.5 at 25°C)
• RNase A
• PA Columns
Quality Control
The quality of the High-Speed Plasmid Advance Kit is tested
on a lot-to-lot basis according to Geneaid's ISO-certified quality management
system. Plasmid DNA from a 50 ml overnight E. coli (DH5α) culture, containing
plasmid pBluescript (A600 > 2 U/ml) is purified using the High-Speed Plasmid
Advance Kit. Following the purification process, a yield of more than 200 μg is
obtained and the A260/A280 ratio is between 1.8-2.0. The purified plasmid (1
µg) is used in EcoRI digestion, and checked by electrophoresis.
High-Speed Plasmid Advance Kit Functional Test Data
Figure 1. Plasmid DNA was extracted using the High-Speed Plasmid Advance Kit. The purified supercoiled plasmid DNA [50 ml overnight E. coli (DH5α) culture, containing a 3 kb plasmid pBluescript (A600 > 2 U/ml)], from a 2 ml eluate was used in EcoRI digestion and analyzed by electrophoresis on a 1% agarose gel. M = Geneaid 1 Kb DNA Ladder |
Download User Manual
Plasmid Purification | |
Prep Size | Midi |
Technology | Glass fiber membrane |
DNA/RNA Purification | |
Yeld up to | 500µg |
- Availability: In Stock
- Model PA025
- Weight: 2.50kg
- Dimensions (L x W x H): 17.00cm x 21.00cm x 15.00cm