Blood DNA Isolation Kit (100 ml)

Advantages (Cat. # GEB100, GEB01K, GEB01K+)

• Isolate high molecular weight DNA for archiving or sensitive downstream applications
• High yield, High quality DNA (A260/A280 = 1.8-2.0)
• Sample: 300 µl of whole blood, 3 ml of whole blood, 10 ml of whole blood, 10 ml of compromised whole blood
• Easily scalable to satisfy larger blood sample volumes
• Minimal hands on time
• Convenient, simple and gentle reagent DNA precipitation method
• Storage: dry at room temperature (15-25ºC), RNase A should be stored at 4ºC for extended periods

Applications

DNA archiving, PCR, AFLP, RFLP/PADP, Southern Blotting, Real-time PCR 

Components

• RBC Lysis Buffer
• Cell Lysis Buffer
• Protein Removal Buffer 
• DNA Hydration Buffer (10 mM Tris-HCL, 1 mM EDTA, pH8.0)
• RNase A (10 mg/ml) - not included with GEB01K

Quality Control

The quality of the Geneaid™ DNA Isolation Kit (Blood) is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Genomic DNA is isolated from 300 µl of fresh whole human blood. The isolated DNA (5-15 µg with an A260/A280 ratio of 1.8–2.0) is quantified with a spectrophotometer and analyzed by electrophoresis.

Geneaid™ DNA Isolation Kit (Blood) Functional Test Data

Figure 1. High molecular weight genomic DNA (100-200 kb) was extracted using both the Geneaid™ DNA Isolation Kit (Blood) and the Gentra® Puregene® Blood Kit. Genomic DNA was extracted from 300 µl of whole human blood and resolved in 100 µl of DNA Hydration Buffer. 5 µl of the resolved genomic DNA was analyzed by electrophoresis on a 1% agarose gel.