The Geneaid™ Midi Plasmid
Kit uses pre-packed anion-exchange resin columns to purify plasmid DNA
from 50-300 ml of cultured bacterial cells. TrueBlue Lysis Buffer (an optional
color indicator) is included with the kit in order to prevent common handling
errors, ensuring efficient cell lysis and neutralization. A modified alkaline
lysis method and RNase treatment are used to obtain clear cell lysate with
minimal genomic DNA/RNA contaminants. Using an efficient gravity-flow
procedure, plasmid DNA is bound to the column and contaminants are efficiently
removed. The purified plasmid DNA is eluted then precipitated with isopropanol
for desalting. The entire procedure can be completed without ultracentrifuges,
HPLC or other toxic reagents and the purified plasmid DNA is suitable for
transfection, sequencing reactions, ligation, PCR, in-vitro transcription,
microinjection, restriction enzyme digestion and gene gun.
Advantages (Cat. # PI025)
- High Yield: >400 µg of pure
transfection grade plasmid DNA from 100 ml of cultured bacterial
cells
- Sample Volume: 50-300 ml of cultured bacterial
cells
- Format: plasmid midi anion-exchange resin
column
- Elution
Volume: 500 µl - 2 ml
- Operation time: within 2 hours (only 80
minutes when combined with the Presto™ Plasmid Concentrator)
- Storage: dry at room temperature (15-25ºC)
Applications
Transfection,
Sequencing, Ligation, PCR, In-vitro Transcription, Microinjection, Restriction
Enzyme Digestion, Gene Gun
Components
- PM1, PM2, PM3 Buffer
- TrueBlue Lysis Buffer
- PEQ Buffer
- PW Buffer
- PEL Buffer
- RNase A
- Plasmid Midi Columns
Quality Control
The quality of the
Geneaid™ Midi Plasmid Kit is tested on a lot-to-lot basis according to
Geneaid's ISO-certified quality management system. Plasmid DNA from
a 100 ml overnight E. coli (DH5α) culture, containing plasmid
pBluescript (A600 > 2 U/ml) is purified using the Geneaid™ Midi
Plasmid Kit. Following the purification process, a yield of more than 400
μg is obtained and the A260/A280 ratio is between 1.8-2.0. The purified
plasmid (1 µg) is used in EcoRI digestion, and analyzed
by electrophoresis.
Download User Manual
Plasmid Purification | |
Endotoxin level | <0.15 EU/µg |
Prep Size | Midi |
Technology | Anion Exchange Resin |
DNA/RNA Purification | |
Yeld up to | 800µg |
- Availability: In Stock
- Model PI025
- Weight: 2.50kg
- Dimensions (L x W x H): 17.00cm x 21.00cm x 15.00cm