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Basic DNA

DNA Sequencing Sample Submission Guidelines

For the most cost-efficient way of your DNA sequencing, please use single PCR tubes, 8-strip, or 96 well plate to submit your DNA sample and your sequencing primers. This way could speed up our DNA sequencing meanwhile save your cost for the service.

You may choose three different formats (single PCR tubes, 8-strip, or 96 well semi skirted plate) as shown on picture below and mark your samples and primers accordingly before submission.

                                                             




When you submit 48 or more samples, the best way to do is to using a 96-well PCR plate. Cap the wells tight with 8-strip caps. To secure your samples from leakage during transportation. We suggest wrapping the plate in parafilm to further secure the strip caps in position.

Sample Preparation

Non-Premixed Samples:
Template TypeDNA ConcentrationDNA VolumePrimer ConcentrationPrimer Volume
Plasmid100 ng/µl10 µl3.2µM10 µl
PCR(100-500bp)2-5 ng/µl10 µl3.2µM10 µl
PCR(500bp-1kb)5-10 ng/µl20 µl3.2µM10 µl
PCR(>1kb)10-20 ng/µl20 µl3.2µM10 µl


To expedite your sequencing process you may arrange your samples vertically (in columns) as shown below and fill out the online order form before submission.

We highly recomend to label your name initials and submission date on the tubes or at the plate edges that you are submitting in order to assist us to server you faster.

  

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