Guanidine Isothiocyanate (100 g)
Appearance: White crystalline powder
Solubility: Water: Freely soluble
Ammonia: Not more than 0.05%
Absorbance: (6M) 280nm : ≤0.5
pH: (6M): 4.5-6.0
Melting Point: 115-122 °C
Loss on Drying: ≤0.3%Extraction of cellular DNA and RNA releases DNase and RNase proteins which function by denaturing DNA and RNA respectively. Guanidine thiocyanate is a preferred chemical to use during cell lysis because of its ability to irreversibly inactivate these degrading nucleases. In addition to its chaotropic properties, guanidine thiocyanate has also shown to inactivate enveloped viruses and some non-enveloped viruses.