Taq DNA polymerase (500 units)

Taq DNA polymerase (500 units)

Cloned Taq DNA Polymerase is expressed and purified from E. coli that carries the Taq DNA Polymerase gene from Thermus aquaticus. Taq DNA polymerase is a thermostable enzyme that catalyzes the polymerization of nucleotides in the 5’-to-3’ direction at 70-74 ºC and exhibits a half-time of 30 minutes at 95 ºC. This enzyme possesses 5’-3' exonuclease activity but not 3'-5' exonuclease activity (proofreading activity). The amplified products are up to 5 kb with 3’ adenosine residues and able to use on TA cloning system directly.

Reaction Buffer (PCR Buffer, 10X):

200 mM  Tris-HCl, (pH 8.4), 500 mM KCl.

Storage Buffer:

20 mM Tris-HCl, pH 8.0, 0.1 mM EDTA, 1 mM DTT,  50% glycerol, stabilizers

Unit Definition:

One unit incorporates 10 nmole of dNTPs into acid insoluble material in 30 minutes at 72 ºC

Download User Manual

PCR
Amplicon Size Up to 5 kb
HotStart No
PCR type Standard

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